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KMID : 0829220110350010051
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Korean Journal of Oral and Maxillofacial Pathology
2011 Volume.35 No. 1 p.51 ~ p.56
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Combination Therapy of Photofrin Mediated Photodynamic Therapy with Cisplatin or Carboplatin
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Lim Hyun-Ju
Oh Chung-Hun
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Abstract
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Photodynamic therapy(PDT) is recently developed as an effective treatment for malignant disease. Carboplatin, a less nephrotoxic analog of cisplatin, has been widely used for the treatment of multiple malignancies. In this study, we investigated the cytotoxic and apoptotic effect of combined modality of photofrin mediated PDT with cisplatin and carboplatin on KB cell human oral cancer cell line in vitro. The a ttached KB cells were incu bated with c isplatin(0.04㎎/㎖) and carboplatin(0.02㎎/㎖) for 24h at 37℃ and followed by photosensitization with photofrin for 6h and laser irradiation with 630nm LED at an intensity of 2.0 J/㎠ for activating photofrin for 15min. Then MTT assay and SYTO 16 green & Propidium iodide (PI) double staining were used respectively to measure the cytotoxicity and nuclear morphology at 24h after PDT. This study demonstrates that the combined modality with carbopaltin resulted in enhanced apoptotic cell death as well as cytotoxic e ffect on KB c ells in vitro, which s uggests the feasibility of combined modality and the possibility o f reducing the effective dosage of photofrin and carboplatin and lowering the side effects on normal cells.
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KEYWORD
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Photodynamic therapy, Light emitting diode, Photofrin, Apoptosis, Cisplatin, Carboplatin
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